Table 1

WWOX intron-exon junctions and PCR and sequencing primers

Exon/ intronStarting position in cDNAExon length (bp)Acceptor splice siteaDonor splice siteaIntronic primers for mutation screening (5′-3′)PCR product size (bp)
11232ACGCCAA/gtaagggggc1F-ACTGGATTTCAGCTTCGTGGTCG334
1R-TCCGTGGGCTGTGCAGGGTC
223365tttttaacag/TCACACCGCAGGAG/gtttgtatgt2F-TTCCCCCTACTTCCTTCTTATATCTGGC373
2R-ATCCTCACTCCACCCTATGATCTCATC
329858tgtgtttcag/ATTTGCCTTGTTGA/gtaagtgtct3F-ATGGTCTTTACTTCTCCCTGGCAC244
3R-ACTTCTGCTAAGATTACAGATACACACTG
4356179ttttgggcag/CCATATAGGAATAG/gtaggctctt4F-AGTTCTTTCAGGTTTAAGGAATAAGC370
4R-TAGATCTAAGTGGATCTCATTATAGCAG
5535107taaaccatag/GGTTCGAAGAATGG/gtaagcgctt5F-ACTTGGGGTAATTTAAGTGGTGCTC296
5R-AACTTTACACACTCCACTGAAATCTCC
664289ctcattgcag/CATAAAGGAATGT/gtgagtgttcc6F-ATTAAACAGGGGAATTCCGAC278
6R-TCTCCCAATTGTGTTCATCTG
7731186ttttttcag/GCCTCTTCCCCATCG/gtgggtttga7F-ACATCCATGGATCCCGAAG318
7R-TGGTATGAGAAAGGGGATAAGTG
8917265tatttttaag/ATTTACAGTCCATG/gtaagagaac8F-TGCACCCAGCATTCCTTAGATTTCC459
8R-ACCAGACTCATGCCCGCAAG
911821060ggatttccag/CAACAGG8F-AAATGACGCCATCTCATCACTCC1259
8R-TGTTTTCCTGGCATCTACGAGAAG
  • a Exonic sequences are indicated in uppercase; intronic sequences are indicated in lowercase.