Table 2

Demarcation of the DMR by bisulfite sequencing analysis: LOH-negative sample 3

The methylation patterns across the antisense promoter and regulatory region were assessed in normal kidney and WT DNAs corresponding to LOH-negative sample 3 (see Fig. 1b <$REFLINK> ). Independent clones A–J are shown on the vertical axis, and CpG dinucleotides assessed are numbered on the horizontal axis. The CpGs are oriented relative to Fig. 1a <$REFLINK> by the KpnI and SpeI sites. Methylated CpGs are indicated by + and unmethylated by− .

CpG probe3433(KpnI) ARR probe
393837363532313029282726252423222120191817161514a13a121110987654321
Normal kidney
A
B
C
Dρb
E+ρb
F++++++++++
G+++++++++++
H++++++++++++
I+++++++++++++
J+++++++++++++++
WT
A
B
C
Dρb
Eρb
F+ρb
G+ρb
H+ρb
I++ρb
J+++
  • a Differentially methylated BstUI site (shown with an asterisk in Fig. 1a <$REFLINK> ).

  • b The CpG was unassessable because of the bisulfite analysis primer used.