Reactivity of CD8+ T cells within and around esophageal tumors
Representative examples for quantitative PCR on tumor (Tm) and adjacent normal mucosa (Nm) samples from patient P1 with i.t. CD8+ T cell infiltrations and patient P2 with p.t. location of CD8+ T cells. Mean values of triplicate analysis are shown. Comparative immunofluorescence staining for patient P1 is shown in Fig. 1 <$REFLINK> . | ||||||
---|---|---|---|---|---|---|
Patient | CD8 | IFN-γ | β-actin | CD8/β-actina | IFN-γ/CD8b | |
CD8+ i.t. | P1:Tm | 30.85 | 34.40 | 20.23 | 1.52 | 1.12 |
P1:Nm | 29.72 | 32.11 | 21.83 | 1.36 | 1.08 | |
CD8+ p.t. | P2:Tm | 33.35 | 35.72 | 27.79 | 1.20 | 1.07 |
P2:Nm | 33.37 | 35.92 | 21.78 | 1.53 | 1.08 | |
PBMCc | 32.19 | 34.97 | 25.22 | 1.28 | 1.09 | |
CD8+ flud | 24.18 | 27.99 | 20.15 | 1.20 | 1.16 |
a Values represent copies of CD8 mRNA per copies of β-actin mRNA.
b Values represent copies of IFN-γ mRNA per copies of CD8 mRNA.
c Controls included unstimulated CD8+ T cells from PBMC.
d Controls included influenza-matrix peptide-specific CD8+ T cells 2 h after peptide restimulation (see also Refs. 17 , 18 , and 20 ) that were generated from PBMC as described in “Materials and Methods.”