Table 2

Reactivity of CD8⁺ T cells within and around esophageal tumors

Representative examples for quantitative PCR on tumor (Tm) and adjacent normal mucosa (Nm) samples from patient P1 with i.t. CD8⁺ T cell infiltrations and patient P2 with p.t. location of CD8⁺ T cells. Mean values of triplicate analysis are shown. Comparative immunofluorescence staining for patient P1 is shown in Fig. 1 <$REFLINK> .
	Patient	CD8	IFN-γ	β-actin	CD8/β-actin^a	IFN-γ/CD8^b
CD8⁺ i.t.	P1:Tm	30.85	34.40	20.23	1.52	1.12
	P1:Nm	29.72	32.11	21.83	1.36	1.08
CD8⁺ p.t.	P2:Tm	33.35	35.72	27.79	1.20	1.07
	P2:Nm	33.37	35.92	21.78	1.53	1.08
PBMC^c		32.19	34.97	25.22	1.28	1.09
CD8⁺ flu^d		24.18	27.99	20.15	1.20	1.16

a Values represent copies of CD8 mRNA per copies of β-actin mRNA.
b Values represent copies of IFN-γ mRNA per copies of CD8 mRNA.
c Controls included unstimulated CD8⁺ T cells from PBMC.
d Controls included influenza-matrix peptide-specific CD8⁺ T cells 2 h after peptide restimulation (see also Refs. 17 , 18 , and 20 ) that were generated from PBMC as described in “Materials and Methods.”