Table 1

Survey of NSCLC cell lines for phosphorylation of Akt/PKB at S473

Lysates were prepared from cells exposed to 10% FBS (HS), 0.1% FBS overnight (LS), or LS + IGF-I (10 nm) for 10 min. Immunoblotting was performed with antibodies against phospho-S473 Akt/PKB and native Akt/PKB. + indicates phosphorylation of Akt/PKB at serine 473. − indicates that there was no detectable Akt/PKB phosphorylation at S473. NT designates samples that were not tested, and * indicates that the histology, status, or expression is unknown.
Phospho-S473 status
NSCLC linesHSLSTumor histologyp53 statusRb expressionKras status
H1703++SQMutWTWT
H157++SQWT*Mut
A549+SQWTWTWT
H1155++LCMutMutMut
H1355ADMutWTMut
H1334++LCMutWTWT
H460++LCWTWTMut
H1299++LCMutWTMut
H727++CAMutWTMut
H838++ADWTWTWT
H2228++ADWTMutWT
H2594++ME*WT*
H2444**WT*
H2009++ADMut*Mut
H2409++AD*WT*
H3072++**WT*
H2850++**WT*
H2973++**WT*
H1374++**WT*