Table 1

Summary of proteins affected after 24-h treatment with 17AAG

Concentrations used were the minimum isoeffective dose sufficient to deplete c-Raf-1 to <95% of the untreated control at 24 h: 0.5 μm for HT29, 1 μm for HCT116 and KM12, and 50 μm for HCT15 cells. +/−, <95% of control; +, <50% of control; ++, equal to control; ND, not detected. All results were replicated in two independent experiments.
c-Raf-1Ki-rasN-rasERK-1/2Phospho-Erk-1/2c-AktGAPDH
HT29
 Control++++++++++++++
 17AAG
  24 h+/−++++++/−++
  48 h+/−+/−+/−+/−+/−+/−++
  72 h+/−+/−+/−+++++
HCT116
 Control++++++++++++++
 17AAG
  24 h+/−++/−++/−+++
  48 h+++++++++/−++
  72 h++++++++++++
KM12
 Control++++++++ND++++
 17AAG
  24 h+++++ND+++
  48 h+++/−+/−ND+++
  72 h+++++ND++++
HCT15
 Control++++++++++++++
 17AAG
  24 h+/−++++++++/−++
  48 h+++++++++
  72 h+++++++++++