Characterization of percentage of cells positive for the CAR, αvβ3, and αvβ5 receptors as measured by FACS analysis and study of infectivity of cells by Ad-CMV-LacZ Vector at 20 MOI as measured by β-galactosidase assay (X-Gal)
| αvβ3, αvβ5, and CAR receptor levels were measured by mouse monoclonal antibodies, and the FITC-conjugated antimouse antibody was used to stain the cells. Then, FACS analysis was used to detect the percentage of the receptor-positive cells (n = 2). For infectivity, cells were exposed to virus in serum-free medium for 90 min at 20 MOI and incubated for 48 h in culture medium. Then, cells were stained by X-Gal analysis (n = 2). | ||||
|---|---|---|---|---|
| αvβ3 | αvβ5 | CAR | β-Gal | |
| EJ | 83 ± 8 | 82 ± 5 | 95 ± 8 | 95 ± 8 |
| J82 | 56 ± 6 | 78 ± 7 | 80 ± 10 | 88 ± 10 |
| Skov-3 | 64 ± 6 | 91 ± 8 | 87 ± 7 | 85 ± 11 |
| Ovcar-5 | 48 ± 7 | 57 ± 5 | 88 ± 10 | 65 ± 8 |
| Hey | 81 ± 5 | 96 ± 10 | 0 | 10 ± 4 |
| CCD | 63 ± 8 | 93 ± 4 | 29 ± 5 | 70 ± 9 |