Table 1

Characterization of percentage of cells positive for the CAR, αvβ3, and αvβ5 receptors as measured by FACS analysis and study of infectivity of cells by Ad-CMV-LacZ Vector at 20 MOI as measured by β-galactosidase assay (X-Gal)

αvβ3, αvβ5, and CAR receptor levels were measured by mouse monoclonal antibodies, and the FITC-conjugated antimouse antibody was used to stain the cells. Then, FACS analysis was used to detect the percentage of the receptor-positive cells (n = 2). For infectivity, cells were exposed to virus in serum-free medium for 90 min at 20 MOI and incubated for 48 h in culture medium. Then, cells were stained by X-Gal analysis (n = 2).
αvβ3αvβ5CARβ-Gal
EJ83 ± 882 ± 595 ± 895 ± 8
J8256 ± 678 ± 780 ± 1088 ± 10
Skov-364 ± 691 ± 887 ± 785 ± 11
Ovcar-548 ± 757 ± 588 ± 1065 ± 8
Hey81 ± 596 ± 10010 ± 4
CCD63 ± 893 ± 429 ± 570 ± 9