Table 1

Metastatic tumor burden in soft tissue organs, as assessed by semiquantitative histomorphometry

No significant differences exist between OPG-treated and untreated groups (one-way ANOVA).
Cell lineOPGOrgana
LiverLungHeartBrainAdrenalOvaryKidneyPancreas
C260.6 ± 0.20.8 ± 0.23.2 ± 0.40.8 ± 0.41.8 ± 0.21.2 ± 0.51.4 ± 0.50
C26+1.0 ± 0.51.8 ± 0.52.4 ± 0.20.2 ± 0.22.0 ± 0.02.0 ± 0.01.2 ± 0.20
MB-MDA-23100.2 ± 0.22.2 ± 0.52.2 ± 1.41.6 ± 0.40.2 ± 0.20.2 ± 0.20.4 ± 0.2
MB-MDA-231+00.6 ± 0.41.4 ± 0.51.4 ± 0.51.0 ± 0.5000
  • a One section of each organ from each mouse (n = 8–10 mice/group) was analyzed by standard light microscopy using a scoring system described in “Materials and Methods.” Data represent means ± SD, n = 10 mice/group. No metastases were observed in the spleen of any animals (data not shown).