Table 2

Effect of iNOS inhibition on 3[H] thymidine incorporation by melanoma cells

Melanoma cell lines (5 × 104/ml) were cultured for 72 h in medium with (0.1%) or without FCS and in the presence of iNOS inhibitor AMG either alone or in combination with SNP.[ 3H] thymidine incorporation was evaluated during the last 18 h of culture. Results are expressed a cpm ± SD. Bold values are significantly different (SNK test, P at least <0.01 and <0.001 in most instances) from [3H] thymidine incorporation observed in medium without AMG.

Melanoma cell lineSerumAMG
0.1 mm0.5 mm2 mm2 mm + SNP (50 μm)
617,768 ± 1,22314,767 ± 1,18812,834 ± 8234,647 ± 51721,963 ± 296
+25,463 ± 1,06025,846 ± 1,21121,547 ± 1,7254,594 ± 51737,528 ± 2,734
107,381 ± 2,2915,036 ± 1,0383,125 ± 1,0591,969 ± 19318,593 ± 2,030
+22,064 ± 1,83418,962 ± 2,10315,817 ± 1,21517,772 ± 4,01069,783 ± 13,148
134,115 ± 1102,779 ± 2252,421 ± 1581,325 ± 3213,617 ± 718
+5,219 ± 5835,062 ± 3854,109 ± 3642,104 ± 6976,025 ± 682
1422,465 ± 4,09016,581 ± 1,23911,984 ± 52011,350 ± 75536,045 ± 1,553
+43,163 ± 6,37335,645 ± 77030,138 ± 3,77428,325 ± 3,26570,777 ± 5,233