Table 1

Transformation of NHA by introduction of E6/E7, hTERT, and Ras

Cell typesColony numbersa% tumor incidence (n)b
NHA0ND
 hTERT0ND
 E7/hTERT0ND
 E6/E7/hTERT0ND
 E6/E7/hTERT/wtEGFR0ND
 E6/E7/hTERT/EGFRvIII11 ± 40 (5)
 E6/E7/hTERT/Akt0ND
 E6/E7/hTERT/wtEGFR/Akt95 ± 310 (5)
 E6/E7/hTERT/EGFRvIII/Akt105 ± 210 (5)
 E6/E7/hTERT/Ras1018 ± 20591 (11)
 E6/E7/hTERT/Ras + PD98059105 ± 31ND
 E6/E7/Ras0ND
 E7/hTERT/Ras0ND
U251MG1455 ± 142100 (5)
  • a Cells (1 × 104) were plated in soft agar, and colonies were scored after 3 weeks. Results represent mean ± SD from two separate experiments.

  • b Cells were injected s.c. into nude mice. n, number of animals treated. ND, not done.