Table 2

Growth properties of in vivo-derived recurrent variants of A431

Cell typeC225-dependent growth inhibition (% ± SD)aTGF-α-dependent growth inhibition (% ± SD)aDoubling time (h ± SD)bClonogenic survivalc (colony, n ± SD)b
A43133 ± 5.691 ± 5.230.2 ± 2.3186 ± 4.5
R141 ± 10.080 ± 3.228.7 ± 0.6189 ± 1.5
R343 ± 5.388 ± 7.930.6 ± 1.1111 ± 6.0
R433 ± 11.485 ± 7.532.4 ± 1.1176 ± 13.2
R535 ± 8.184 ± 9.435.4 ± 1.658 ± 7.1
  • a Values represent the average for two independent experiments, each one including triplicates.

  • b Calculated from triplicate cultures.

  • c The ability of cells to form clonal colonies was analyzed as described previously (58) . See “Materials and Methods” for details.