Table 2

Effect of CV706, radiation, or both on necrosis, apoptosis, and vascularization in LNCaP tumor xenografts

LNCaP tumor-bearing animals were treated with CV706 (i.t. administration of 1 × 107 particles/mm3 of tumor), radiation (local tumor radiation at a dose of 10 Gy), or both as described in “Materials and Methods.” Tumor samples were harvested 14 days after treatment and analyzed by histological staining (H&E for necrosis and blood vessel and immunohistochemical staining with anti-CD31 antibody for angiogenesis) and TUNEL assay (for apoptotic cells). Groups consisted of six mice each.
TreatmentNecrosisBlood vesselCD31+ cellsApoptosis
Vehicle5.4 ± 2.287.5 ± 6.333.7 ± 8.24.7 ± 1.2
CV706258.2 ± 80.858.5 ± 3.114.8 ± 3.924.6 ± 7.2
Radiation67.1 ± 48.227.5 ± 8.920.6 ± 7.38.9 ± 2.2
CV706 + Radiation461.6 ± 37.94.5 ± 1.98.4 ± 2.778.3 ± 12.2