Table 2

Effect of rPAI-1 on α5β1 and active β1 expression in HBMECs

HBMEC cells were plated on BSA, vitronectin, or fibronectin-coated wells in the presence or absence of rPAI-1 (100 nm) for 1 h. Cells were then harvested in dissociation buffer and analyzed for α5β1 expression and active β1 expression by FACS analysis. The data represent the percentage of the cell population with a signal above background.
% of cells expressing
Conditionsα5β1Active β1
BSA83.955.3
Vitronectin85.063.9
Vitronectin + PAI-185.264.7
Fibronectin85.062.1
Fibronectin + PAI-186.363.9