Table 3

Recognition of substituted MAGE-A10254–262 peptide variants by MAGE-A10-specific T cells

LAU 50/15 (0.13 nm)aLAU 50/22 (0.21 nm)LAU 155/6D1 (0.23 nm)LAU 119/Pb (0.25 nm)LAU 203/1B8 (0.05 nm)LAU 169/4E8 (92 nm)LAU 42/2D6 (58 nm)HD 591/P (0.28 nm)HD 795/2B6 (2 nm)HD 795/4D11 (42 nm)
Peptide sequencec
 GLYDGMEHL1d111111111
ALYDGMEHL20.0029<0.00012<0.000151.2350
 GAYDGMEHL0.40.720.10.40.010.30.002101.2
 GLADGMEHL0.0020.0010.005<0.0001<0.0001<0.0001<0.00010.0007<0.0001<0.0001
 GLYAGMEHL0.0020.0012<0.00010.001<0.0001<0.00010.0007<0.0001<0.0001
 GLYDAMEHL0.00040.0001<0.0001<0.00010.1<0.0001<0.0001<0.0001<0.0001<0.0001
 GLYDGAEHL0.00010.00020.001<0.0001<0.0001<0.0001<0.0001<0.0001<0.0001<0.0001
 GLYDGMAHL0.010.00060.004<0.00010.0018<0.0001<0.0001<0.0001<0.0001
 GLYDGMEAL732160.00021<0.000116<0.00013
 GLYDGMEHA0.050.006<0.00010.10.04<0.000140.04<0.0001<0.0001
  • a Numbers in parentheses represent the nanomolar concentration of parental MAGE-A10254–262 peptide required for 50% maximal lysis by the corresponding T-cell population in a standard 4-h chromium release assay.

  • b P, polyclonal.

  • c Alanine substitutions are in bold.

  • d Relative antigenic activity of single A-substituted MAGE-A10254–262 peptide variants as compared with MAGE-A10254–262. The relative antigenic activity for each peptide compared with that of the parental peptide was calculated as described in “Materials and Methods.”