Table 3

Recognition of substituted MAGE-A10_254–262 peptide variants by MAGE-A10-specific T cells

	LAU 50/15 (0.13 nm)^a	LAU 50/22 (0.21 nm)	LAU 155/6D1 (0.23 nm)	LAU 119/P^b (0.25 nm)	LAU 203/1B8 (0.05 nm)	LAU 169/4E8 (92 nm)	LAU 42/2D6 (58 nm)	HD 591/P (0.28 nm)	HD 795/2B6 (2 nm)	HD 795/4D11 (42 nm)
Peptide sequence^c
GLYDGMEHL	1^d	1	1	1	1	1	1	1	1	1
ALYDGMEHL	2	0.002	9	<0.0001	2	<0.0001	5	1.2	3	50
GAYDGMEHL	0.4	0.7	2	0.1	0.4	0.01	0.3	0.002	10	1.2
GLADGMEHL	0.002	0.001	0.005	<0.0001	<0.0001	<0.0001	<0.0001	0.0007	<0.0001	<0.0001
GLYAGMEHL	0.002	0.001	2	<0.0001	0.001	<0.0001	<0.0001	0.0007	<0.0001	<0.0001
GLYDAMEHL	0.0004	0.0001	<0.0001	<0.0001	0.1	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001
GLYDGAEHL	0.0001	0.0002	0.001	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001
GLYDGMAHL	0.01	0.0006	0.004	<0.0001	0.001	8	<0.0001	<0.0001	<0.0001	<0.0001
GLYDGMEAL	7	3	2	16	0.0002	1	<0.0001	16	<0.0001	3
GLYDGMEHA	0.05	0.006	<0.0001	0.1	0.04	<0.0001	4	0.04	<0.0001	<0.0001

^a Numbers in parentheses represent the nanomolar concentration of parental MAGE-A10_254–262 peptide required for 50% maximal lysis by the corresponding T-cell population in a standard 4-h chromium release assay.
^b P, polyclonal.
^c Alanine substitutions are in bold.
^d Relative antigenic activity of single A-substituted MAGE-A10_254–262 peptide variants as compared with MAGE-A10_254–262. The relative antigenic activity for each peptide compared with that of the parental peptide was calculated as described in “Materials and Methods.”