Table 1

Sequences of primers/probes used for quantitative RT-PCR

The primers and probes were designed with Primer Express (ABI-Perkin-Elmer) using the Genbank Accession number, which appears in parentheses.
GeneOligonucleotide sequence
IGF-I (M27544)Forward5′-AAGTCAGCTCGCTCTGTCCG-3′
Reverse5′-TTCCTGCACTCCCTCTACTTGC-3′
Probe5′-TCTGGGTCTTGGGCATGTCGGTGT-3′
IGF-IR (X04434)Forward5′-GTGAAAGTGACGTCCTGCATTTC-3′
Reverse5′-CCTTGTAGTAAACGGTGAAGCTGA-3′
Probe5′-CACCACCACGTCGAAGAATCGCATC-3′
IGFBP-3 (X64875)Forward5′-CGCCAGCTCCAGGAAATG-3′
Reverse5′-GCATGCCCTTTCTTGATGATG-3′
Probe5′-CAGCACGCACCGGGTGTCTGATC-3′
IGFBP-5 (M62782)Forward5′-AAAGAGCTACCGCGAGCAAG-3′
Reverse5′-GGAGATGCGGGTGTGTTTG-3′
Probe5′-ACGAGGAGCCCACCACCTCTGAGA-3′
rRNA (M10098)Forward5′-CGGCTACCACATCCAAGGAA-3′
Reverse5′-GCTGGAATTACCGCGGCT-3′
Probe5′-TGCTGGCACCAGACTTGCCCTC-3′