Table 1

Molecular analysis of aggressive cutaneous melanoma cells

ProteinSymbolFunctionRatioa
Laminin (γ2)LAMCBasement membrane (ECM)50b, 33c, 22.5d
MMP-1MMP1Proteinase, digests ECM (interstitial collagenase)36; ND; ND
MMP-2MMP2Proteinase, digests ECM (gelatinase)11.7; 18.5; 9.1
MMP-9MMP9Proteinase, ECM (gelatinase)2.7; 2.2; 2.5
MT-1 MMPMMP14Membrane-associated collagenase (activates MMP2/TIMP-2 complex)2.2; 2.6; 3.5
TIMP-1TIMP1Tissue inhibitor of matrix metalloproteinase-14.9; 3.1; 6.9
TIMP-2TIMP2Tissue inhibitor of matrix metalloproteinase-20.37; 0.36; 2.5
  • a cDNA microarray analysis of altered gene expression: relevant expression of selected genes is reported as the ratio of highly invasive/metastatic to poorly invasive/nonmetastatic cells.

  • b NIHGRI array results.

  • c Metastasis Chip array results. For both arrays, RNA was isolated from cell cultures, labeled by incorporation of Cy3-dCTP (less aggressive, C81-61 cells) or Cy5-dCTP (highly aggressive, C8161 cells), pooled, and hybridized onto one array. Bacillus subtilis clones dap, phe, lys, and thr were used to normalize fluorescence hybridization signals for the Metastasis Chip array.

  • d Metastasis Chip array results for more aggressive MUM-2B cells vs. less aggressive MUM-2C cells, as described above in c.