Table 1

Relationship between the sensitivity of human colorectal cancer cells to topoisomerase inhibitors and their status of MMR and p53

Cell linesSensitivity toaMMR statusp53 statusG2-M arrestb
CPTETP
CaCo2ProficientWild type++
HT29ProficientArg273His+++
SW480+ProficientArg273His/Pro309SerNDc
LoVo++++++hMSH2−Wild type+
HCT116++++++hMLH1−, hMSH3−Wild type+++
SW48+++++hMLH1−Wild type++
DLD1+++hMSH6−Wild type/Ser241Phe++
  • a The sensitivity to CPT was scored as follows: −, IC50 ≥ 50 nm; +, 20 nm < IC50 < 50 nm; ++, 10 nm < IC50 < 20 nm; +++, IC50 < 10 nm. The sensitivity to ETP was scored as follows: −, IC50 ≥ 10 μm; +, 5μ m ⩽ IC50 < 10μ m; ++, 1 μm < IC50 < 5 μm; +++, IC50 < 1μ m.

  • b G2-M arrest was scored as the percentage of cells in G2-M as follows: +, <35%; ++, 35–70%; +++, >70%.

  • c ND, not determined.