Table 1

Summary of the in vitro phenotypic characteristics of the BPH-1-derived lineages

Confluent and subconfluent (subconf) cells were stained immunocytochemically to determine expression and localization of epithelial markers.
E-cadherinβ-cateninPan keratinHMW keratinp63Keratin 8Vimentin
Parental BPH-1+++a++++++++++++
Parental BPH1 (subconf)+++++ (membrane)+++
BPH1CAFTD-01++++ (membrane)++++++++−/+
BPH1CAFTD-01 (subconf)+++ (membrane and cytoplasm)++++
BPH1CAFTD-02+++ (membrane)+++++++++−/+
BPH1CAFTD-02 (subconf)+++/−+++−/+
BPH1CAFTD-03+/−+/−++++++++++
BPH1CAFTD-03 (subconf)+/−+/−+++
BPH1CAFTD-04+/−+ (cytoplasm)++++++++++
BPH1CAFTD-04 (subconf)+ (cytoplasm)+++
BPH1CAFTD-05+++/−++++++++++
BPH1CAFTD-05 (subconf)+/−+/−+++
BPH1CAFTD-06+/−+/−++++++++++
BPH1CAFTD-06 (subconf)+++/−+++
BPH1CAFTD-07+/−++ (membrane)++++++++++−/+
BPH1CAFTD-07 (subconf)+/−++ (membrane)+++−/+
BPH1CAFTD-08++++++++++
BPH1CAFTD-08 (subconf)+++++
BPH1TETD-A+/−−/+ (cytoplasm)+++++++++−/+
BPH1TETD-A (subconf)+/−−/+ (cytoplasm)++++
BPH1TETD-B+/−++ (membrane)++++++++
BPH1TETD-B (subconf)+/−−/+ (cytoplasm)+++−/+
  • a Staining: −, absent; −/+, weak (minority of cells); +/−, weak (majority of cells); +, uniform weak; ++, moderate; +++, strong.