Table 1

Verification of DMH results by Southern blot analysis

Patient no.CpG island loci
SC13B2SC76F1CpG15E5CpG6H12CpG5H12CpG18A1SC77F6
SouthernaDMHbSouthernDMHSouthernDMHSouthernDMHSouthernDMHSouthernDMHSouthernDMH
25+3.0+2.0+5.9[−1.6]+2.4+2.01.2
31+4.1+1.8+10.8+2.2+5.6+2.61.1
43[−1.5]c1.4ND0.7[−3.8]+2.61.0
451.4+5.1ND1.0+5.2+5.91.4
65+3.0+1.8+1.8ND1.1+2.21.2+2.7
83+2.11.2+2.1+3.3+3.21.00.7
91[−1.5]1.3+4.6+3.0+3.7+3.50.5
1091.11.21.10.91.01.10.8
119+1.7+1.7+ND0.9[+1.2]+2.31.2
129+1.71.31.41.3+1.6+1.71.4
151[+1.4]1.1+1.81.2+1.90.6+1.8
155+4.2+1.8+7.2+2.7+7.8[−3.3]ND1.8
1570.91.2+1.61.3[+1.1]0.8+2.7
159+4.0+2.3+3.8+2.2+4.7+4.40.5
167+NDd+1.6+1.90.8+2.0+2.4+2.4
241+2.5+4.6+9.8+1.7+6.3+1.60.5
243+1.8+2.0+2.81.2+4.0+2.80.7
  • a Southern blot visual scores: +, hypermethylation in tumor DNA; −, no methylation differences between tumor and normal DNA. See Fig. 2 <$REFLINK> for selected examples.

  • b Adjusted DMH Cy5:Cy3 ratios. As described in the text, the cutoff ratio for hypermethylation in tumor DNA is set at ≥1.5.

  • c Loci with ratios enclosed in brackets depict those not confirmable by Southern analysis.

  • d ND, not determined.