Table 1

Semiquantitative RT-PCR of WIBC-9 and non-IBC xenograftsa

Target genesWIBC-9MC-2MC-5MC- 18SK- BR3Activated lymphocyte
EGFR family
EGFR +++++
ErbB-2 ++
ErbB-3 +++++
ErbB-4 +++++
Angiogenic factors
VEGF ++++
VEGF-C ++
VEGF-D ++
ANG-1 ++++++
ANG-2 +
bFGF +++++
Acid FGF ++
TGF-α ++
TGF-β1 ++++
VEGF receptors
flt-1 (mouse)+++++
flk-1 (mouse)++++
flt-4 (mouse)++++
Flt-1 +++++
KDR +++++
Angiopoietin receptors
tie-1 (mouse)+++++
tie-2 (mouse)+++
Tie-1 +
Tie-2 +
Cytokines
IL-1β +
IL-6 +
IL-8 +++++++
IL-10 +
IFN-γ +
M-CSF ++++++
Adhesion molecules
Integrin αv+++++
Integrin αv (mouse)++++++
Integrin β3++++++
Integrin β3 (mouse)+++++
Intercellular adhesion molecules
E-cadherin+++++
VE-cadherin (mouse)+++
  • a Expression of genes for epidermal growth factors, angiogenic factors, cytokines, and EC adhesion molecules was assayed with RT-PCR. The relative expression levels were calculated as the density of the products divided by that of each internal control (− 0.3, 0.3 ≦ + < 1.0, ++≥ 1.0). M-CSF, macrophage colony-stimulating factor.