Table 1

Effect of c-kit antisense oligonucleotides on SCF-induced anchorage-independent growth of DLD-1 cellsa

Culture conditionsColony numbers (1 × 103 cells/well)
Medium59 ± 11b
SCF128 ± 29
Medium + c-kit S ODN63 ± 12
SCF + c-kit S ODN118 ± 34
Medium + c-kit AS ODN44 ± 19
SCF + c-kit AS ODN73 ± 29c
  • a DLD-1 cells were exposed to the culture conditions indicated for 10 days. DLD-1 cells were pretreated with c-kit sense (S) and antisense (AS) oligonucleotides (ODN) as described in “Materials and Methods.”

  • b Significantly different from control (SCF + c-kit S ODN, P = 0.03) as determined by Student’s t test.

  • c Values represent mean ± SD of three separate experiments, each consisting of triplicate cultures.