Table 1

Immunohistochemistry controls

SamplesStaining pattern
Control cell lines
 ASPC-1 (negative control)No staining
 MDA PCa 2b (positive control)Nuclear staining
Control tissues
 Human testicle (MKK4 positive by Western)Epithelial-specific staining
 Rat testicle (MKK4 positive by Western)Epithelial-specific staining
 Normal human prostate (n = 16)Epithelial-specific staining
Immunohistochemical controls
 Isotype controlNo staining
 Epitope controlNo staining
 Secondary antibody onlyNo staining
 Endogenous peroxidase/avidin biotinNo staining