Table 1

NQO and GST activities in the cytosolic fraction of mouse small intestine

DietNQO activityaGST activitya
Nrf2(+/+)Nrf2(−/−)Nrf2(+/+)Nrf2(−/−)
Control 1156 ± 6 (100)71 ± 7 (46)43 ± 0.2 (100)31 ± 0.2 (72)
BHA742 ± 40 (475)b93 ± 6 (59)b116 ± 6.0 (270)b43 ± 0.5 (100)b
Control 2165 ± 4 (100)74 ± 5 (45)53 ± 0.8 (100)36 ± 0.4 (68)
EQ1026 ± 76 (622)b86 ± 4 (52)c245 ± 6.0 (462)b50 ± 1.0 (94)b
OPZ212 ± 9 (128)b77 ± 6 (47)39 ± 1.0 (74)26 ± 0.5 (49)
I3C238 ± 12 (170)b89 ± 4 (54)c71 ± 0.1 (134)b31 ± 0.3 (58)
Control 3164 ± 3 (100)75 ± 1 (45)32 ± 0.2 (100)28 ± 0.1 (88)
C+K293 ± 6 (179)b66 ± 3 (40)78 ± 0.6 (244)b23 ± 0.1 (72)
SUL237 ± 15 (145)b57 ± 4 (35)49 ± 0.4 (153)b20 ± 0.3 (63)
Control 4250 ± 20 (100)141 ± 40 (56)35 ± 0.3 (100)29 ± 0.4 (85)
CMRN617 ± 50 (247)b132 ± 40 (53)65 ± 0.5 (181)b39 ± 0.2 (111)b
3-OH CMRN328 ± 50 (131)92 ± 30 (37)41 ± 0.2 (117)b29 ± 0.2 (85)
7-OH CMRN310 ± 40 (124)122 ± 30 (49)37 ± 0.2 (106)30 ± 0.7 (86)
LMTN332 ± 30 (133)c95 ± 40 (38)52 ± 0.6 (153)b28 ± 0.2 (80)
AGLN770 ± 70 (308)b109 ± 40 (44)77 ± 0.4 (220)b35 ± 0.4 (100)b
  • a Activity is expressed as nmol/min/mg for both NQO and GST. The values presented represent mean catalytic activities (±SE) from triplicate measurements of pooled intestinal cytosol from three separate animals. The numbers in parentheses represent the mean activity value as a percentage of the value obtained with Nrf2(+/+) on a control diet within the same experimental group.

  • b Significantly greater from control of same genotype, P < 0.01.

  • c Significantly greater from control of same genotype, P < 0.05.