Table 2

Quantitative analysis of NQO1 protein in intestinal cytosol from Nrf2(+/+) and Nrf2(−/−) mice given dietary supplements

DietDensitometric estimation of protein levelsa
Nrf2(+/+)Nrf2(−/−)
Control 110032.3 (100)
BHA72469.7 (216)
Control 210019.6 (100)
EQ43454.2 (277)
OPZ19837.4 (191)
I3C24433.3 (170)
Control 310027.4 (100)
C+K15241.3 (150)
SUL11510.0 (37)
Control 410030.8 (100)
CMRN20863.2 (205)
3-OH CMRN14620.5 (67)
7-OH CMRN12721.9 (71)
LMTN12715.3 (50)
AGLN25518.8 (61)
  • a The level of NQO1 was calculated from densitometric scans of the Western blot autoradiography film. The results are typical of those from at least three separate analyses of intestinal cytosol combined from three mice. The data presented are calculated as a percentage of the value obtained from the appropriate group of Nrf2(+/+) mice on the RM1 control diet. Those results shown in parentheses are calculated as a percentage of the value obtained from Nrf2(−/−) mice fed on the control diet.