Table 1

Binding capacities to immunopurified HLA-DR molecules NY-ESO-1 peptides

IC₅₀s are expressed in nm and have been evaluated from at least three independent experiments. The biotinylated peptides used at indicated concentrations in these assays are the following: HA 306–318 (PKYVKQNTLKLAT) for DR1 (1 nm), DR4 (30 nm), DR11 (40 nm), and DRB5 (10 nm); MT 2–16 (AKTIAYDEEARRGLE) for DR3 (200 nm); YKL (AAYAAAKAAALAA) for DR7 (5 nm); B1 21–36 (TERVRLVTRHIYNREE) for DR13 (500 nm); A3 152–166 (EAEQLRRAYLDGTGVE) for DR15 (30 nm); Lo1 191–120 (ESWGAVWRIDTPDKLTGPFT) for DRB3 (10 nm); and E2/E168 (AGDLLAIETDKATI) for DRB4 (3 nm). IC₅₀s for the active peptides are <1000 nm and are indicated in bold (19) .