Table 1

Binding capacities to immunopurified HLA-DR molecules NY-ESO-1 peptides

IC50s are expressed in nm and have been evaluated from at least three independent experiments. The biotinylated peptides used at indicated concentrations in these assays are the following: HA 306–318 (PKYVKQNTLKLAT) for DR1 (1 nm), DR4 (30 nm), DR11 (40 nm), and DRB5 (10 nm); MT 2–16 (AKTIAYDEEARRGLE) for DR3 (200 nm); YKL (AAYAAAKAAALAA) for DR7 (5 nm); B1 21–36 (TERVRLVTRHIYNREE) for DR13 (500 nm); A3 152–166 (EAEQLRRAYLDGTGVE) for DR15 (30 nm); Lo1 191–120 (ESWGAVWRIDTPDKLTGPFT) for DRB3 (10 nm); and E2/E168 (AGDLLAIETDKATI) for DRB4 (3 nm). IC50s for the active peptides are <1000 nm and are indicated in bold (19) .

Allele frequencyAlleles
DR1DR3DR4DR7DR11DR13DR15DRB3DRB5DRB4
Peptides
 NY-ESO-1 119–143 20 >10,000 12 49 5 >10,000 40 800 43 125
 NY-ESO-1 119–133 220 >10,000 13 63 1630>10,000 400 1,8308,3301,830
 NY-ESO-1 121–138 65 >10,000 20 13 38 >10,000>10,0003,000 600 >10,000
 NY-ESO-123–137 18 >10,000 27 11 11 >10,000>10,0001,250 775 >10,000
 NY-ESO-1 129–1431,670>10,0001050 333 550 >10,0008,000>10,000 550 175