Table 1

MSP assay for RARβ gene

Cell lineRARβ mRNARARβ methylation
Squamous cell carcinoma
Large cell carcinoma
Anaplastic carcinoma
Bronchioloalveolar cell carcinoma
Adenosquamous carcinoma
Small cell carcinoma
  • MSP analysis was performed on lung cancer cell lines with different histopathological features. Cell lines were characterized as sensitive (+) or resistant (−) to the effects of RA on RARβ mRNA levels. MSP was performed using methylated- and unmethylated-specific primers, to identify cell lines with (Pos) and without (Neg) RARβ promoter methylation. The experiment was performed twice and produced the same results each time.