Table 1

MSP assay for RARβ gene

Cell lineRARβ mRNARARβ methylation
Squamous cell carcinoma
 Calu-1Neg
 H226BNeg
 H226BrPos
 SK-MES-1Pos
Adenocarcinoma
 H322Neg
 H358Pos
 H441Pos
Large cell carcinoma
 H460Pos
 H661+Neg
 H1299+Neg
Anaplastic carcinoma
 Calu-6+Neg
Bronchioloalveolar cell carcinoma
 A549Neg
Adenosquamous carcinoma
 H596Pos
Small cell carcinoma
 H1607Pos
  • MSP analysis was performed on lung cancer cell lines with different histopathological features. Cell lines were characterized as sensitive (+) or resistant (−) to the effects of RA on RARβ mRNA levels. MSP was performed using methylated- and unmethylated-specific primers, to identify cell lines with (Pos) and without (Neg) RARβ promoter methylation. The experiment was performed twice and produced the same results each time.