Table 1

Strains and plasmids used in this study

StrainGenotypeSource
MHY340 MATa/α ura3-52/ura3-52 leu2Δ1/leu2Δ1 his3Δ200/his3Δ200 ade2/ADE2 ade3/ADE3 lys2LYS2 trp1/TRP1 M. Henry (UMDNJ)
BMY66 MATa ura3-52 leu2Δ1 his3Δ200 trp1 This study
BMY100as MHY340 Δdut1::HIS3/DUT1This study
BMY101as BMY66 Δdut1::HIS3+ pBAM10This study
BMY102as BMY66 Δdut1::HIS3+ pBAM11This study
BMY106as BMY66 Δdut1::HIS3+ pBAM11+ pPS293This study
BMY107as BMY66 Δdut1::HIS3+ pBAM11+ pBAM12This study
BMY108as BMY66 Δdut1::HIS3+ pBAM11+ pBAM13This study
BMY109as BMY66 Δdut1::HIS3+ pBAM11+ pBAM14This study
BMY114as BMY102 Δung1::TRP1+ pPS293This study
BMY119as BMY102 Δung1::TRP1+ pBAM12This study
PlasmidRelevant markersSource
pRS315Ampr CEN6 LEU2M. Henry
pRS316Ampr CEN6 URA3M. Henry
pPS293Ampr 2μ URA3 GAL1 promoterM. Henry
pBAM10Ampr CEN6 URA3—hDUT-NThis study
pBAM11Ampr CEN6 LEU2—hDUT-NThis study
pBAM12Ampr 2μ URA3 GAL1—DUT1This study
pBAM13Ampr 2μ URA3 GAL1—UNG1This study
pBAM14Ampr 2μ URA3 GAL1—UgiThis study