Table 2

Expression levels of selected proteins in matched normal ductal/lobular breast cells and DCIS

ProteinaCasebTrendDifferential spot intensitycReported function(s)
Cytoskeletal regulation
 Macrophage capping protein (CapG)4DCIS>Nd1.9Blocks actin barbed ends, dissociates with PIP2
 CapZ5DCIS>N22.9«
 Profilin 15DCIS>N34.4Binds PIP2, monomeric actin
 Cofilin4DCIS>N24.8Binds PIP2, actin turnover
 Actin-related protein 3 (Arp3)5DCIS>N3.3Actin nucleation
 Transgelin (Basic)2(I/C)N>DCIS1.4Actin spatial organization
4N>DCIS
 Transgelin (Acidic)2DCIS>(I/C)N2.2
4DCIS>N
l-Plastin4DCIS>N17.7Actin bundling
 Coactosin-like protein4DCIS>N69.5F-actin binding
 Stathmin4DCIS>N136.8Microtubule destabilization
Lipid and vesicular trafficking
 Rab 11a3DCIS>N20.6Vesicle recycling, trafficking from trans-Golgi exocytosis
 Annexin II3DCIS>N2.6Lipid rafts, Secretion Vitamin D responses
 Annexin V1DCIS>N5.8Membrane trafficking, linkage to cytoskeleton inhibition of PKC, pLAC
3DCIS>N
 Annexin VII1DCIS>N4.0Vesicle fusion, Secretion
Subcellular trafficking
 Cellular retinoic acid-binding protein 2 (CRABP2)5DCIS>N5.0Transport of Retinoic Acid to RAR
4DCIS>
 Ubiquitin-conjugating enzyme 9 (Ubc9)4DCIS>N, LCMNuclear envelope targeting of RanGAP1
 Intracellular chloride channel 1 (NCC27, CLIC1)5DCIS>N, LCM3.3Nuclear and plasma membrane ion transport
6DCIS>N, LCM
 Selenium binding protein4N>DCIS46.3Intra-Golgi protein transport
 Polymeric immunoglobulin receptor (pIgR)4N>DCIS4.1Transport of dimeric IgA across epithelial cells
 Phosphoethanolamine binding protein (PEBP), prostatic binding protein2(I/C) N>DCIS, LCM N>DCIS, LCM2.1Recruitment of signaling proteins to membrane, immunosuppressive factor
 Fatty acid binding proteinFatty acid intracellular transport
  FABP-3 (HFABP)4N>DCIS3.6
  FABP-5 (EFABP)4DCIS>N1.2
Chaperones
 Hsp 272DCIS>N, LCM21.5Protein conformation, stabilization
3DCIS>N
4*DCIS>N, LCM
 Hsp 904DCIS>N26.1Protein conformation, stabilization, intracellular trafficking, degradation. Steroid receptor activation
 GRP945*DCIS>N1.7Secretory and membrane protein chaperone, binds Her-2, peptides
 GRP786DCIS>N1.5Endoplasmic reticulum, protects secretory proteins, binds peptides, MHC
 Prohibitin3DCIS>NNDMitochondrial protein conformation, degradation
 α B-crystallin4DCIS>N14.1Protein conformation, stabilization
Extracellular matrix
 Type VI collagenSupercoiled extracellular matrix, cell adhesion
  α1 chain4N>DCIS, LCM2.4
  α2 chain1N>DCIS3.2
4N>DCIS, LCM
Protease related
 Cathepsin B3DCIS>NNDProtease
 Annexin II3DCIS>N2.6Cell surface binding of Procathepsin B, tissue plasminogen activator
 α-Enolase5DCIS>N1.7Plasminogen receptor
 Phosphoethanolamine-binding protein (PEBP), prostatic-binding protein2(I/C) N>DCIS, LCM2.1Also a serine protease inhibitor
4N>DCIS, LCM
 Anti-thrombin III5N>DCIS4.0Fibrinolytic system
Apoptosis
 Voltage-dependent anion Channel protein 1 (VDAC)4N>DCIS, LCM4.3eMitochondrial receptor for Bax, Bak
1.2
 Peroxiredoxin 1 (Thioredoxin peroxidase)1DCIS>N454Cytoplasmic antioxidant
 Peroxiredoxin 23DCIS>N8.5«
 Peroxiredoxin 52(I/C) N>DCIS, LCM3.6fMitochondrial antioxidant
LCM1.6
Translation
 Eukaryotic translation initiation factor eIF3h4N>DCIS1.1Translation
 Elongation factor 1α5DCIS>N, LCM14.1Translation
 Nascent polypeptide-associated complex, a polypeptide4N>DCIS, LCMNDBinds nascent polypeptides
Signaling
 Protein phosphatase 2A (PP2A)4DCIS>N1.6Growth regulation, stimulatory and inhibitory effects reported
 14-3-3 ζ/δ5DCIS>N3.7Multiple aspects of signal transduction
 Rho GDI6DCIS>N21.5Rho, Rac function
 FK506 binding protein2(I/C) N>DCIS1.6EGF receptor phosphorylation, calcium channel function
Nuclear function
 Ubiquitin-conjugating enzyme 9 (Ubc9)4DCIS>N9.6Alters transcription from Ets, Tel, androgen receptor
 HnRNPsPre-mRNA splicing, transport; binds PKC; telomere maintenance
  A24DCIS>N2.1«
  A34DCIS>N1.2«
  C4DCIS>NND«
  L*4DCIS>NND«
  X4DCIS>N3.3«
  A2/B16DCIS>N, LCM1.3«