Table 2

Metastasis assay

Colon 26 cells (5 × 104) were injected into the spleen of WT and TNF-Rp55-deficient mice. Mice were sacrificed at 24 days after tumor injection to determine the incidence of liver metastasis, the numbers of metastatic foci in the liver, and tumor sizes. Tumor sizes were calculated by an equation of a × b2/2, where a and b indicate long and short diameters of the tumor, respectively. The parameters of liver weight and tumor size were determined in mice, which developed liver metastases. Each value represents mean ± SE.

TNFRp55 KOWTP
Liver metastasis
 Incidence12/26 (46.2%)24/26 (92.3%)0.002
 Weight (g)1.47 ± 0.191.70 ± 0.270.012
 Volume (mm3)147.0 ± 131.8287.9 ± 186.40.026
 (Range, median)(8.0–507.5, 108.0)(94.5–671.5, 220.5)
 Maximum (mm)5.0 ± 1.97.1 ± 1.80.003
 Number2.9 ± 1.74 ± 1.80.1
Spleen tumor
 Diameter (mm)14.2 ± 4.412.9 ± 4.90.31
 Weight (g)1.11 ± 0.151.03 ± 0.210.29