Table 1

PCR primers sequences, annealing temperatures, and PCR product sizes used for MSP

GeneForward primer (5′ → 3′)Reverse primer (5′ → 3′)Annealing temperature (°C)Size (bp)
p16 = MaTTATTAGAGGGTGGGGCGGATCGCGTGCACCCGACCCCGAACCGCGACCGTAA64150
p16 = UTTATTAGAGGGTGGGGTGGATTGTCAACCCCAAACCACAACCATAA60151
DAPK = MGGATAGTCGGATCGAGTTAACGTCCCCTCCCAAACGCCGA5698
DAPK = UGGAGGATAGTTGGATTGAGTTAATGTTCAAATCCCTCCCAAACACCAA61108
GSTP1 = MTTCGGGGTGTAGCGCTCGTCGCCCCAATACTAAATCACGACG5891
GSTP1 = UGATGTTTGGGGTGTAGTGGTTGTCCACCCCAATACTAAATCACAACA5997
  • a M, methylated-specific primers; U, unmethylated-specific primers.