Table 2

Mutations in BCRP/Bcrp1 codon 482 in drug-resistant cell lines

Altered nucleotides are underlined.

cDNA sourceSelecting drugCodon 482Encodes
Human
 placentaaAGGR
 MCF-7/AdrVpadoxorubicin, 3 μMACGT
 S1-M1-80amitoxantrone, 80 μMGGGG
Mouse
 liveraAGGR
 MEF3.8AGGR
 MEF3.8/M32mitoxantrone, 32 nMAGGR
 MEF3.8/T6400topotecan, 6400 nMAGGR
 KOT52AGGR
 KOT52/D80doxorubicin, 80 nMAGGR
 KOT52/D320doxorubicin, 320 nMAGG/ATGR/M: 70/30%
 KOT52/D800doxorubicin, 800 nMAGG/ATGR/M: 40/60%
 88.6AGGR
 88.6/D200-Adoxorubicin, 200 nMAGGR
 88.6/D800-Adoxorubicin, 800 nMATGM
 88.6/D200-Bdoxorubicin, 200 nMAGGR
 88.6/D800-Bdoxorubicin, 800 nMAGTS
  • a From Refs. (1 2 3 , 12 , 13 , 19) and GenBank records.