Table 3

Drug resistance conferred by ectopic expression of Bcrp1 in MEF3.8 cells

Shown are the IC50s (in nM), determined by n assays and the resistance factors for a representative Bcrp1-transduced clone. Values shown here for the MEF3.8 parent line may differ somewhat from the earlier determinations shown in Table 1 <$REFLINK> because of variations in drug batches and details of the assay procedure. Results of two-tailed heteroscedastic t tests comparing the IC50s of the parent and transduced lines are shown.

DrugMean IC50 ± SD, nM, and (n)RFt test
MEF3.8MEF3.8/Bcrp1 clone A2
Mitoxantrone0.40 ± 0.10 (6)19 ± 2 (5)48P < 0.01
Doxorubicin3.6 ± 0.3 (3)13 ± 1.2 (3)3.6P < 0.01
Topotecan45 ± 7 (4)1800 ± 100 (3)40P < 0.01
Vincristine0.25 ± 0.10 (4)0.26 ± 0.01 (3)1.0nsa
  • a ns, not significant.