Table 1

Flow cytometry analysis for the expression of CCR7 and CD45RA on CD8+ T cells directed to gp100209–217, Melan-A/Mart-126–35 and Influenza-Matrix58–66 epitopes in PBL, TILN, and TFLN of melanoma patients

TissuenaCCR7/CD45RA phenotype of gated CD8+ tetramer+ T cells directed tobFrequency of tetramer T cells directed to
gp100209–217Melan-A/Mart-126–35Flu-Matrix58–66gp100209–217Melan-A/Mart-126–35Flu-Matrix58–66
+/++/−−/−−/++/++/−−/−−/++/++/−−/−−/+
PBL1121 (5–43)13 (2–27)25 (13–35)40 (17–58)37 (19–54)15 (4–36)18 (6–30)30 (12–59)26 (10–44)12 (4–34)25 (10–36)38 (21–50)0.347 (0.038–1.132)0.194 (0.058–0.464)0.203 (0.044–0.906)
cTILN76 (1–11)19 (7–42)71 (50–83)4 (1–8)2 (0–5)9 (2–17)87 (76–98)1 (0–3)8 (3–20)7 (1–12)72 (40–86)14 (6–30)0.499 (0.082–2.332)7.063 (1.502–23.285)0.179 (0.021–0.528)
cTILN1665 (26–93)18 (7–51)8 (0–28)9 (0–35)69 (28–96)16 (4–33)9 (0–44)6 (0–37)70 (37–97)19 (3–40)6 (0–20)5 (0–25)0.144 (0.012–0.667)0.250 (0.043–0.810)0.109 (0.023–0.289)
TFLN828 (13–45)27 (4–47)22 (4–38)23 (4–45)51 (32–66)23 (14–34)15 (0–35)12 (0–17)31 (13–45)26 (3–47)21 (9–35)22 (2–45)0.260 (0.073–0.606)0.362 (0.123–0.892)0.443 (0.086–1.280)
  • a Percentage of positive cells (mean value, with range in brackets) for each of the four possible CCR7/CD45RA phenotypes is reported. nd, not done.

  • b Frequency of tetramer+ T cells (mean value, with range in brackets) is expressed as % of positive cells/CD8+ lymphocytes.

  • c The two groups of TILN (7 patients versus 16 patients) identified by high versus low frequency to Melan-A/Mart-1 epitope differ for the proportion of tetramer+ T cells expressing a CCR7+/CD45RA+ and a CCR7 CD45RA phenotype (P < 0.001).