Table 3

Flow cytometry analysis for the expression of perforin and CCR7 in CD8+ T cells directed to Melan-A/Mart-126–35 and MAGE-3271–279 from TILN and TFLN of melanoma patients

TissueN#aCCR7/Perforin phenotype of gated CD8 tetramer T cells directed to
Melan-A/Mart-126–35MAGE-3271–279
+/++/−−/−−/++/++/−−/−−/+
bTILN48 (5–11)8 (2–20)17 (5–29)67 (40–85)8 (3–15)90 (84–95)1 (0–4)1 (0–5)
bTILN87 (2–15)80 (40–97)12 (0–53)2 (0–10)10 (5–16)89 (83–95)1 (0–3)0 (0–1)
TFLN56 (3–8)86 (74–93)6 (0–19)2 (0–11)11 (2–27)87 (71–98)1 (0–3)1 (0–2)
  • a Percentage of positive cells (mean values with range in brackets) for each of the four possible CCR7/perforin phenotypes is reported.

  • b The two groups of TILN (4 patients versus 8 patients) were identified by high versus low frequency to Melan-A/Mart-1 epitope.