Table 1

Ovarian neoplasms induced by a single intrabursal administration of AdCMVCre to mice carrying conditional p53 and/or Rb1 alleles

Genotypep53floxP/floxPp53floxP/floxP Rb1floxP/floxPRb1floxP/floxPWild-type
Mice with neoplasms, total, %a13 (4/31)b100 (34/34)3 (1/29)0 (0/32)
Mice with ovarian neoplasms, total %6 (2/31)97 (33/34)0 (0/29)0 (0/32)
Well-differentiated serous epithelial neoplasms of the ovary, %0 (0/2)39 (13/33)0 (0/0)0 (0/32)
Poorly differentiated CK8-positive neoplasms of the ovary, %100 (2/2)45 (15/33)0 (0/0)0 (0/0)
Undifferentiated neoplasms of the ovary, %c0 (0/2)15 (5/33)0 (0/0)0 (0/0)
Peritoneal spreading, %0 (0/2)27 (9/33)0 (0/0)0 (0/0)
Ascites, %0 (0/2)24 (8/33)0 (0/0)0 (0/0)
Metastases in the contralateral ovary, %0 (0/2)15 (5/33)0 (0/0)0 (0/0)
Lung metastases, %50 (1/2)18 (6/33)0 (0/0)0 (0/0)
Liver metastases, %0 (0/2)3 (1/33)0 (0/0)0 (0/0)
  • a Nonovarian neoplasms were not physically associated with the ovary and included poorly differentiated spindle and polymorphic cell neoplasms without any morphological or immunohistochemical features of specific differentiation, such as expression of epithelial (CK8 and Pan-cytokeratin), lymphoid (CD45R and CD3), smooth (smooth muscle actin), and striated (α-sarcomeric actin) muscle and histiocytic (F4/80) and schwannian (S100) markers. All of the neoplasms had no functional floxed gene(s) according to microdissection PCR genotyping and were likely a result of rare inadvertent leakage of the AdCre during intrabursal administration.

  • b Numbers in parentheses indicate number of mice with neoplasm out of total number of mice.

  • c Neoplasms originating from the ovary but without any specific morphological or immunohistochemical features of differentiation (see comment to a). All of the neoplasms had loss of both floxed genes according to PCR genotyping.