Table 2

Kinetics of Stat3 binding to phosphododecapeptides determined by mirror resonance biosensor analysis

PhosphododecapeptideKD (nm)akass (m s)bkdiss (ms)ct1/2 (min)d
Y1068135 ± 20e,f1894 ± 6420.23 ± 0.0558.4 ± 12.1
Y1086243 ± 36f2361 ± 10580.61 ± 0.3431.2 ± 19.1
  • a Dissociation equilibrium constant determined from biosensor equilibrium data.

  • b Association rate constant determined from slope of line from plot of kon versus ligand.

  • c Dissociation rate constant determined from KD multiplied by kass.

  • d Half-life of dissociation, which equals ln2 divided b kdiss.

  • e Mean ± SE of 2–4 separate experiments.

  • f P = 0.044.