Table 2

Growth properties of the transfectants

Cell linesGrowth in monolayersa
Doubling time (h)Saturation density (104 cells)
NIH3T319.57.7 ± 0.6
V-1519.87.0 ± 2.1
UbcH10-1715.714.9 ± 0.7
UbcH10-2014.918.0 ± 2.7
  • a Doubling time is determined by calculating the growth rate of exponentially growing cells. Saturation density is the number of cells/well after the culture had reached confluence (means ± SE). Each experiment was carried out at least twice.