Table 1

Inhibition of EGF-induced tyrosine phosphorylation in transfected 3T3 cells

EGF-stimulated phosphotyrosine content was evaluated after a 15-min stimulation with 50 ng/ml EGF to the indicated cell line as described in “Materials and Methods.” Inhibitors at the indicated concentrations were pre-incubated with the cells for 2 h prior to stimulation. Mean ± SD is indicated for quadruplicate determinations.

InhibitorConcentration (μm)Phosphotyrosine content % of EGF-stimulated control
EGFr transfectantErbB2/EGFr chimera transfectant
None (Control)(DMSO vehicle)100 ± 4100 ± 3
Tarceva0.131 ± 0.589 ± 2
Tarceva0.320 ± 0.474 ± 2
Tarceva17 ± 0.546 ± 2
Tarceva3<57 ± 5
CP-6545770.1101 ± 123 ± 0.5
CP-6545770.374 ± 66 ± 1
CP-654577131 ± 12<5
CP-654577314 ± 2<5