Table 1

Functional grouping of genes identified by cDNA microarray analysis as being up-regulated by 5-FU in MCF-7 cells

Potential target genes were grouped according to their function using the DRAGON database.

FamilyExamplesFold inductionSignal intensity
Signal transductionRaf3.98686
K-ras4.89662
SLAP5.05391
Phosphoinositide 3-kinase3.2918
ApoptosisCOP9 homolog (HCOP9)8.61587
Apoptosis specific protein4.61625
APO-1 cell surface antigen4.24453
FLIP protein3.75793
Cell cycleCyclin G8.513789
CDC23.11779
Cyclin-dependent protein kinase-25.93416
StructuralThymosin β-108.527041
Myosin light chain3.2397
Gelsolin7.318482
Thymosin β-44.346355
Polyamine metabolismSSAT13.03662
Spermidine synthase3.73874
Spermidine aminopropyltransferase5.06633
Cell surfaceMAT-8 protein10.16522
Annexin II12.324463
Annexin IV9.34101
FGFa receptor 24.9684
Transmembrane 4 superfamily protein3.2491
MitochondrialChaperonin 1011.68478
Enoyl-CoA hydratase3.42512
Nicotinamide nucleotide transhydrogenase4.71508
Ribosomal proteinsRibosomal protein S2810.924039
Ribosomal protein L373.0723
L23 mRNA for putative ribosomal protein4.612662
Ribosomal protein L75.51724
  • a FGF, fibroblast growth factor.