Table 2

Analysis of tumors arising in B6↔BR chimeric mice

Tumors were induced by i.p. injection with DEN (0.05 μmol/gram body weight) at 12 days of age. Males were sacrificed at 30–34 weeks and females at 50–52 weeks. Tumors were excised and frozen on dry ice.

No. of tumorsNo. analyzedOrigin of tumorsaDegree of liver chimerism
BRB6Ind.b% B6 (GPI)c
Males
 1d2397e2f0>80
 2g98332>80
 3d5615933>80
 4d63161510>80
 5485500>80
 684181800>80h
 7d31413075
 8d,g6417170050
 9g8431050
 10d95121110<20
 11d,g387700<20
 12d97242400<20
 13d,g71202000<20
 14169711<20
 15g70171412<20
Total [Mean ± SD]7731851472018[52 ± 25]
Females
 1214301>80
 2g154400>80
 3601291250
 4d8210150
 512214140030h
 6g121121200<20
 745191900NDi
Total [Mean ± SD]392676214[52 ± 29]
  • a The strain of origin of tumors was determined by analyzing DNA from tumors using a quantitative PCR assay. Three different SSLP markers were used to type each tumor DNA sample. Tumors were designated as derived from BR if >65% of the tumor DNA was BR, B6 if >65% of tumor DNA was B6, and indeterminate if DNA was not >65% derived from BR or B6.

  • b Indeterminate.

  • c Unless otherwise noted, the degree of chimerism in the liver was determined by comparing the relative activity of GPI-A with GPI-B.

  • d Mixed sex chimera as determined by the Zfx:Zfy ratio.

  • e Male by Zfx:Zfy ratio.

  • f Female by Zfx:Zfy ratio.

  • g R26 chimera.

  • h The degree of chimerism in the liver was determined by analyzing DNA from non-neoplastic areas using the quantitative PCR assay used to type tumors.

  • i Not determined.