Table 1

IRF-5-modulated genes involved in cell cycle arrest and apoptosis

IRF-5-responsive genes were identified by microarray analysis using the SuperArray GEArray Q series gene arrays. RNA isolated from BJAB cells or HCT116 p53−/− cells expressing IRF-5 was used for these studies. Levels of induction or repression were determined by statistical analyses using background subtractions and data normalization with control genes on each microarray. The table lists genes of interest that were modulated by IRF-5. Text in bold represents genes confirmed by RPA and/or immunoblot analysis. The asterisk denotes p53 target genes (36 , 52) . a (+) ≥2.5–4.99-fold induction; (++) ≥5.0–9.99-fold induction; (+++) ≥10.0; (−) ≤0.0. The data are representative of two to three independent microarray gene array experiments. The number(s) of IRF-E consensus sequence(s) in the promoter is indicated, except in those cases where sequences have not been deposited in GenBank (NA, not available).

Gene nameGenbank no.Induction (+) Repression (−)No. of IRF-E consensus sequence (promoter/coding region)
Bak1 AA923639 ++ NA
p21Waf1 (p21Cip1)* L47233 + 4
Bax* L22474 + 1
Caspase 6* U20537 + NA
Caspase 8 NM_001228 ++ 0
TNFRSF12 (DR3)U74611+NA
TNFRSF8 (CD30)NM_001243++1
TNFRSF10B (DR5)*AF016266+3
DAP-kinase 2 NM_014326 ++ NA
Chk2 (RAD53)NM_007194+NA
Cyclin G2L49506+NA
CyclinB1 M25753 2
Cdc2 (cdk1)NM_0017860
Bcl-2* M14745 NA
Survivin (API4)U752850
  • a B. J. Barnes, unpublished data.