Table 2

Mitotic defects induced by treatment with BMI-1026

Mitotic phenotypesa% cells
Normal mitosis4.3% (n = 2)
Normal metaphase alignment; cut morphology41.3% (n = 19)
No metaphase alignment; cut morphology54.3% (n = 25)
Total numbern = 46
  • a U-2 OS cells expressing GFP-histone H2B were treated with 50 nm BMI-1026. Time-lapse microscopy was carried out to examine various mitotic defects for up to 48 h after treatment. Chromosomal DNA morphologies were determined by visualizing GFP-histone H2B signals.