Table 1

Effect of FK866 on NAD(P)+ synthesis rate (pmol/mg protein/6 h) of HepG2 cellsa

PrecursorTreatmentNicotinic acidNicotinamideNAD(P)+
NicotinamideVehicle23 ± 410 ± 2370 ± 25
Nicotinamide8 h 10 nm FK86621 ± 316 ± 211 ± 2
  • a HepG2 cells (4 × 106/10 ml) were cultured with 10 nm FK866 or vehicle for 8 h. During the last 6 h of the incubation period, 14[C]nicotinamide was added. NAD(P)+ synthesis was quantified in cellular extracts by chromatography and subsequent autoradiography. Results expressed as mean ± SD (n = 3).