Table 1

Expression of HLA class I and HLA-DR antigens in glioma cell lines in the absence or presence of IFN-γa

HLA class IHLA-DR
SFISFImRNA
− IFN-γ+ IFN-γ− IFN-γ+ IFN-γ− IFN-γ+ IFN-γ
A17221.917.21.06.30.0000.176
T98G191.1202.21.0117.80.0008.449
LN-229134.3271.9193.5303.73.07112.941
D247MG79.0148.01.05.50.0000.739
LN-42819.640.91.014.40.0000.169
U138MG38.152.61.01.10.0001.584
LN-1822.043.91.05.30.0001.156
U251MG16.433.61.05.80.0001.509
LN-3085.213.51.01.00.0000.043
U373MG105.0240.01.45.30.0005.831
LN-31913.321.52.431.00.0001.010
U87MG8.015.81.910.10.0000.254
  • a Protein expression of HLA class I and HLA-DR antigens was quantified by flow cytometry using anti-HLA class I mAb W6/32 and anti-HLA-DR mAb L243 and expressed as SFI [modified from (18)] . mRNA expression of HLA-DR-α was quantified by relative QRT-PCR. TNF-α-matured DCs were used as a positive control and set to 100% (data not shown). Data are expressed as a percentages relative to this positive control.