Table 2

Genes that uniquely change in the LR5 cell line compared with the drug-sensitive cells in suspension that could impact DNA repair, glutathione synthesis, melphalan transport, and cell cycle progression

Shown is the fold change ± SD as well as the mean signal for each condition (n = 4). No significant changes were observed in FN-adherent cells compared with suspended cells in these categories. Method of statistical comparisons is described in detail in the “Materials and Methods.”

LR5 fold changeSignalDescription
SusaLR5
DNA damage
1.76 ± 0.82229360Ligase III, DNA, ATP-dependent
1.78 ± 0.2411221994RAD51 homologue C
2.70 ± 0.25282751FANC, complementation group F
1.65 ± 0.45276449UV-radiation resistance-associated gene
0.40 ± 0.1717063BRCA1-associated protein
Cell cycle
3.97 ± 3.70129545Cyclin-dependent kinase inhibitor 1C (p57Kip2)
1.46 ± 0.217911135Cyclin-dependent kinase inhibitor 1B (p27Kip1)
0.82 ± 0.25257210Cyclin D1 (parathyroid adenomatosis 1)
0.85 ± 0.97438241CDC25A
Amino acid transport
1.62 ± 0.71420632Putative l-type neutral amino acid transporter
0.82 ± 0.3439973087Solute carrier family 7 (cationic amino acid transporter system)
Glutathione
1.67 ± 0.409241505Glutamate-cysteine ligase, catalytic subunit
  • a Sus, suspended cells.