Table 2

Genes that uniquely change in the LR5 cell line compared with the drug-sensitive cells in suspension that could impact DNA repair, glutathione synthesis, melphalan transport, and cell cycle progression

Shown is the fold change ± SD as well as the mean signal for each condition (n = 4). No significant changes were observed in FN-adherent cells compared with suspended cells in these categories. Method of statistical comparisons is described in detail in the “Materials and Methods.”

	LR5 fold change	Signal		Description
	LR5 fold change	Sus^a	LR5	Description
DNA damage
	1.76 ± 0.82	229	360	Ligase III, DNA, ATP-dependent
	1.78 ± 0.24	1122	1994	RAD51 homologue C
	2.70 ± 0.25	282	751	FANC, complementation group F
	1.65 ± 0.45	276	449	UV-radiation resistance-associated gene
	0.40 ± 0.17	170	63	BRCA1-associated protein
Cell cycle
	3.97 ± 3.70	129	545	Cyclin-dependent kinase inhibitor 1C (p57Kip2)
	1.46 ± 0.21	791	1135	Cyclin-dependent kinase inhibitor 1B (p27Kip1)
	0.82 ± 0.25	257	210	Cyclin D1 (parathyroid adenomatosis 1)
	0.85 ± 0.97	438	241	CDC25A
Amino acid transport
	1.62 ± 0.71	420	632	Putative l-type neutral amino acid transporter
	0.82 ± 0.34	3997	3087	Solute carrier family 7 (cationic amino acid transporter system)
Glutathione
	1.67 ± 0.40	924	1505	Glutamate-cysteine ligase, catalytic subunit

a Sus, suspended cells.