Table 3

Plasma anti-rMETase-specific antibody titers induced by naked or PEGylated rMETase in mice

IgG and IgM anti-rMETase antibody titers of the plasma of the normal BALB/c mice immunized with naked or indicated PEGylated rMETase in the presence of FCA were determined using ELISA as described in “Materials and Methods.” The numbers in parentheses represent the numbers of mice.

IgGIgM
Naked rMETase10−8 (5)10−4 (4), 10−5 (1)
PEG/rMETase 3010−7 (4), 10−6 (1)10−3 (5)
PEG/rMETase 6010−6 (5)10−3 (5)
PEG/rMETase 12010−4 (4), 10−5 (1)10−3 (5)