Table 1

Ras and BRAF mutations in melanoma cell lines

Cell lineaStageN-RasbH-RasbK-RasbBRAF (exon 11)cBRAF (exon 15)c
FOM74Normald
SBcl2RGP
WM1789RGPA1798G (K600E)
WM35RGP
WM793VGPT1796A (V599E)
WM902BVGPT1796A (V599E)
WM39VGPT1796A (V599E)
WM75VGPT1796A (V599E)
WM278VGPT1796A (V599E)
WM1361AVGP
WM1361BVGPT1796A (V599E)
WM1361CMetastaticT1796A (V599E)
WM983AVGPT1796A (V599E)
WM983BMetastaticT1796A (V599E)
WM1158MetastaticT1796A (V599E)
WM9MetastaticT1796A (V599E)
WM1617MetastaticT1796A (V599E)
1205LuMetastaticT1796A (V599E)
451LuMetastaticT1796A (V599E)
  • a For more information about the cell lines, see Refs. 11 and 12 .

  • b Exons 1 and 2 of N-ras, H-Ras, and K-Ras were amplified by genomic PCR and sequenced.

  • c Exons were screened by CSCE followed by sequencing.

  • d –, no mutation detected.