Table 2

p16 promoter methylation status in normal breast specimens

Patient identificationAge (yr)MSPaMSP-ISHb
969827++
530856++
1081126++
764331+
828518+c
1043432+
450869+
962416+
1175522
151445NDd
1082446ND
1299321ND
946840ND
827533ND
510545ND
1096626ND
1207530ND
1261031ND
1113932ND
1101825NDe
Median age (yr)31
 Methylated3126.5
 Unmethylated36.530.5
Samples methylated (%)7/15 (47)4/14 (29)
  • a MSP scoring: −, unmethylated product but not methylated product detected; +, methylated product detected.

  • b MSP-ISH scoring: −, no positively stained nuclei present in entire section; +, positive nuclei detected.

  • c Few epithelial cells present in section.

  • d ND, not done.