Table 2

ENU-induced mutagenesis at the Hprt gene

GenotypeNo. of HPRT mutants/106 cells
0 mm ENU2.5 mm ENU − O6benzylGa2.5 mm ENU + O6benzylG
Experiment 1
Msh2+/+072
Msh2−/−105186168
Msh2low/−03475
Experiment 2b
Msh2+/+0/15/30/0
Msh2−/−78/94207/210287/275
Msh2low/−5/353/4480/86
Experiment 3b
Msh2+/+0/00/19/11
Msh2−/−66/64295/154257/284
Msh2low/−4/740/3752/38
  • a O6benzylG, O6-benzylguanine.

  • b 106 cells were plated in duplicate.