Table 3

Spectrum of Hprt gene mutations induced by ENU in Msh2-low cells

Mutation+ ENU − O6benzylGa+ ENU + O6benzylG
PositionChangeNo.bPositionChangeNo.b
GC → AT232Leu → Phe1295Gly → Arg2
295Gly → Arg2
722Gly → Gln1
AT → CG182Leu → Trp1710Ile → Met1
AT → TA233Leu → His1151Phe → Ile1
257Met → Lys1158Ile → Lys1
689Asp → Val2287Val → Glu1
710Ile → Asn1386Ile → Asn2
681Val → Asp1
AT → GC620Phe → Ser1233Leu → Pro1
689Asp → Gly1290Leu → Pro1
479Leu → Ser2
689Asp → Gly1
+G490Frameshift1
UnknownSplicecSplice
Exon 21Exon 21
Exon 61Exon 74
Exon 72Exon 9;−17 bpd3
Exon 82
  • a O6benzylG, O6-benzylguanine.

  • b Number of specific mutants found.

  • c Complete exons were deleted from the cDNA, probably reflecting mutations at splice sites.

  • d Cryptic splicing of exon 9; deletion of the first 17 base pairs [as described by Jansen et al. (27)] .