Table 2

Linkage data of the (NTX-10 × O20)F2 and (O20 × NTX-20)F2 crosses

Assay (LPS in ng/ml; duration in hours)a(NTX-10 × O20) or (O20 × NTX-20)bP value (P) and corrected P (CP)cSexdMarif1Marif2Marif3
D4Mit12 or D4Mit54eD8Mit3 or D8Mit125fD19Mit60g
TNF-α (1; 6h)NTX-10P0.00270.00530.000696
CP 0.043
NTX-20P0.0280.0120.00051
CP 0.034
NTX-10 and NTX-20P0.000780.000390.00046
CP 0.021 0.0032
TNF-α (100; 6 h)NTX-10P0.000860.000620.00029
CP 0.039 0.021
NTX-20P0.0160.0065
NTX-10 and NTX-20P0.0000020.00031<0.000001
CP 0.00031 0.017 <0.000001
IL-12 (1; 6 h)NTX-10 and NTX-20P0.0000030.00030
CP 0.00026 0.016
IL-12 (100; 6 h)NTX-10P0.0093
NTX-10 and NTX-20P0.0000830.036
CP 0.0052
IL-12 (100; 24 h)NTX-10P0.0110.0048
NTX-20P0.0110.011
NTX-10 and NTX-20P0.000570.0000170.0400.0096
CP 0.040 0.0012
iNOS (100; 24 h)NTX-10P0.000300.000720.00130.025
CP 0.030 0.042
NTX-20P0.0057
NTX-10 and NTX-20P<0.0000010.000027<0.0000010.020
CP <0.000001 0.0019 0.000031
IL-12 (1 versus 100; 24 h)NTX-10P0.000480.0045
CP 0.028
NTX-10 and NTX-20P0.0000130.000007
CP 0.00095 0.00054
  • a This column lists the type of inflammation-related assay, the amount of LPS used to stimulate BMMf (1 ng/ml or 100 ng/ml), and the duration of stimulation before the assay was performed (in hours). Assays: tumor necrosis factor (TNF-α), secretion of TNF-α; IL-12, secretion of IL-12p40; iNOS, expression of iNOS; IL-12 (1 versus 100), differential effects of low and high concentrations of lipopolysaccharide (LPS) on secretion of IL-12p40.

  • b Data represent statistical evaluation of the (NTX-10 × O20)F2 cross (NTX-10) the (O20 × NTX-20)F2 cross (NTX-20); or the combination of both crosses (NTX-10 and NTX-20).

  • c Suggestive linkage represented by nominal P values < 0.05 (P) and genome-wide significant linkage represented by corrected P values < 0.05 (CP), according to the rules of Lander and Kruglyak (28) .

  • d Effects of sex of the mice on inflammation-related assays. Data represent P values.

  • e Effects of Marif1 on inflammation-related assays. P values listed for the (NTX-10 × O20)F2 cross were obtained using the marker D4Mit12, whereas P values listed for the (O20 × NTX-20)F2 cross and for the combination of both crosses were obtained using the marker D4Mit54.

  • f Effects of Marif2 on inflammation-related assays. P values listed for the (NTX-10 × O20)F2 cross were obtained using the marker D8Mit3, whereas P values listed for the (O20 × NTX-20)F2 cross and for the combination of both crosses were obtained using the marker D8Mit125.

  • g Effects of Marif3 on inflammation-related assays. All P values were obtained using the marker D19Mit60.